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| Covelo-soto, Lara; Moran, Paloma; Pasantes, Juan J.; Perez Garcia, Maria Concepcion. |
| This work explores both the chromatin loss and the differential genome methylation in the sea lamprey (Petromyzon marinus) from a molecular cytogenetic point of view. Fluorescent in situ hybridization experiments on meiotic bivalents and mitotic chromosomes corroborate the chromatin loss previously observed during the development of the sea lamprey and demonstrate that the elimination affects not only to Germ1 sequences but also to the rpt200 satellite DNA and most part of the major ribosomal DNA present on the germinal line. 5-Methylcytosine immunolocation revealed that the GC-rich heterochromatin is highly methylated in the germ line but significantly less in somatic chromosomes. These findings not only support previous observations about genome... |
| Tipo: Text |
Palavras-chave: Sea lamprey; Chromatin loss; Genome rearrangements; DNA methylation. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00247/35811/34327.pdf |
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| Perez Garcia, Maria Concepcion; Rouxel, Julien; Akcha, Farida. |
| In this work, the DNA-damaging effect of hydrogen peroxide on the structural integrity of nucleolar organizer regions (NORs) was studied for the first time by comet-FISH in the Pacific oyster Crassostrea gigas. Global DNA damage was assessed in hemocytes using an alkaline version of the comet assay. Next, NOR sensitivity was analysed by mapping major rDNA repeat unit by fluorescence in situ hybridisation (FISH) on the same comet slides. Exposure of hemocytes to 100 μM of hydrogen peroxide induced a significant increase in both DNA damage and number of FISH-signals of major ribosomal genes versus the control. Moreover, a significant positive correlation was shown between DNA damage as measured by the comet assay (percentage of DNA in comet tail) and the... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Hydrogen peroxide; Comet assay; Comet-FISH; Genotoxicity. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00250/36157/34712.pdf |
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